peripheral blood lymphocyte matchmaker cdna library (TaKaRa)
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peripheral blood lymphocyte matchmaker cdna library
Peripheral Blood Lymphocyte Matchmaker Cdna Library, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 122 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peripheral+blood+lymphocyte+matchmaker+cdna+library/Human+Blood%2C+Peripheral+Leukocytes+Total+RNA/pmc04524097-50-114-120
Average 94 stars, based on 122 article reviews
Peripheral Blood Lymphocyte Matchmaker Cdna Library, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 122 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peripheral+blood+lymphocyte+matchmaker+cdna+library/Human+Blood%2C+Peripheral+Leukocytes+Total+RNA/pmc04524097-50-114-120
Average 94 stars, based on 122 article reviews
peripheral blood lymphocyte matchmaker cdna library - by Bioz Stars,
2026-09
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Construct:Article Title: Role of Host Cell p32 in Herpes Simplex Virus 1 De-Envelopment during Viral Nuclear Egress Article Snippet: Plasmids were constructed for this study as follows. (i) To construct pcDNA-MEF-UL47 , an expression plasmid for UL47 fused to an MEF tag (MEF-UL47), the UL47 ORF without a start codon, was amplified by PCR from pBC1007 ( 52 ) and cloned into pcDNA-MEF ( 53 ). (ii) pcDNA-MEF-gB, an expression plasmid for gB fused to an MEF tag (MEF-gB), was constructed by cloning a DNA fragment encoding MEF-gB amplified by PCR from recombinant virus DNA expressing MEF-gB ( 53 ) into pcDNA4/HisMax C (Invitrogen). (iii) pGEX-p32, for generating a fusion protein of glutathione S -transferase (GST) and p32, was constructed by amplifying the entire p32 coding sequence by PCR from an EBV-transformed human Article Title: Role of Host Cell p32 in Herpes Simplex Virus 1 De-Envelopment during Viral Nuclear Egress Article Snippet: Plasmids were constructed for this study as follows. (i) To construct pcDNA-MEF-UL47 (Fig. 2A), an expression plasmid for UL47 fused to an MEF tag (MEFUL47), the UL47 ORF without a start codon, was amplified by PCR from pBC1007 (52) and cloned into pcDNA-MEF (53). (ii) pcDNA-MEF-gB, an expression plasmid for gB fused to an MEF tag (MEF-gB), was constructed by cloning a DNA fragment encoding MEF-gB amplified by PCR from recombinant virus DNA expressing MEF-gB (53) into pcDNA4/ HisMax C (Invitrogen). (iii) pGEX-p32, for generating a fusion protein of glutathione S-transferase (GST) and p32, was constructed by amplifying the entire p32 coding sequence by PCR from an EBV-transformed human Expressing:Article Title: Role of Host Cell p32 in Herpes Simplex Virus 1 De-Envelopment during Viral Nuclear Egress Article Snippet: Plasmids were constructed for this study as follows. (i) To construct pcDNA-MEF-UL47 , an expression plasmid for UL47 fused to an MEF tag (MEF-UL47), the UL47 ORF without a start codon, was amplified by PCR from pBC1007 ( 52 ) and cloned into pcDNA-MEF ( 53 ). (ii) pcDNA-MEF-gB, an expression plasmid for gB fused to an MEF tag (MEF-gB), was constructed by cloning a DNA fragment encoding MEF-gB amplified by PCR from recombinant virus DNA expressing MEF-gB ( 53 ) into pcDNA4/HisMax C (Invitrogen). (iii) pGEX-p32, for generating a fusion protein of glutathione S -transferase (GST) and p32, was constructed by amplifying the entire p32 coding sequence by PCR from an EBV-transformed human Article Title: Role of Host Cell p32 in Herpes Simplex Virus 1 De-Envelopment during Viral Nuclear Egress Article Snippet: Plasmids were constructed for this study as follows. (i) To construct pcDNA-MEF-UL47 (Fig. 2A), an expression plasmid for UL47 fused to an MEF tag (MEFUL47), the UL47 ORF without a start codon, was amplified by PCR from pBC1007 (52) and cloned into pcDNA-MEF (53). (ii) pcDNA-MEF-gB, an expression plasmid for gB fused to an MEF tag (MEF-gB), was constructed by cloning a DNA fragment encoding MEF-gB amplified by PCR from recombinant virus DNA expressing MEF-gB (53) into pcDNA4/ HisMax C (Invitrogen). (iii) pGEX-p32, for generating a fusion protein of glutathione S-transferase (GST) and p32, was constructed by amplifying the entire p32 coding sequence by PCR from an EBV-transformed human Plasmid Preparation:Article Title: Role of Host Cell p32 in Herpes Simplex Virus 1 De-Envelopment during Viral Nuclear Egress Article Snippet: Plasmids were constructed for this study as follows. (i) To construct pcDNA-MEF-UL47 , an expression plasmid for UL47 fused to an MEF tag (MEF-UL47), the UL47 ORF without a start codon, was amplified by PCR from pBC1007 ( 52 ) and cloned into pcDNA-MEF ( 53 ). (ii) pcDNA-MEF-gB, an expression plasmid for gB fused to an MEF tag (MEF-gB), was constructed by cloning a DNA fragment encoding MEF-gB amplified by PCR from recombinant virus DNA expressing MEF-gB ( 53 ) into pcDNA4/HisMax C (Invitrogen). (iii) pGEX-p32, for generating a fusion protein of glutathione S -transferase (GST) and p32, was constructed by amplifying the entire p32 coding sequence by PCR from an EBV-transformed human Article Title: Role of Host Cell p32 in Herpes Simplex Virus 1 De-Envelopment during Viral Nuclear Egress Article Snippet: Plasmids were constructed for this study as follows. (i) To construct pcDNA-MEF-UL47 (Fig. 2A), an expression plasmid for UL47 fused to an MEF tag (MEFUL47), the UL47 ORF without a start codon, was amplified by PCR from pBC1007 (52) and cloned into pcDNA-MEF (53). (ii) pcDNA-MEF-gB, an expression plasmid for gB fused to an MEF tag (MEF-gB), was constructed by cloning a DNA fragment encoding MEF-gB amplified by PCR from recombinant virus DNA expressing MEF-gB (53) into pcDNA4/ HisMax C (Invitrogen). (iii) pGEX-p32, for generating a fusion protein of glutathione S-transferase (GST) and p32, was constructed by amplifying the entire p32 coding sequence by PCR from an EBV-transformed human Amplification:Article Title: Role of Host Cell p32 in Herpes Simplex Virus 1 De-Envelopment during Viral Nuclear Egress Article Snippet: Plasmids were constructed for this study as follows. (i) To construct pcDNA-MEF-UL47 , an expression plasmid for UL47 fused to an MEF tag (MEF-UL47), the UL47 ORF without a start codon, was amplified by PCR from pBC1007 ( 52 ) and cloned into pcDNA-MEF ( 53 ). (ii) pcDNA-MEF-gB, an expression plasmid for gB fused to an MEF tag (MEF-gB), was constructed by cloning a DNA fragment encoding MEF-gB amplified by PCR from recombinant virus DNA expressing MEF-gB ( 53 ) into pcDNA4/HisMax C (Invitrogen). (iii) pGEX-p32, for generating a fusion protein of glutathione S -transferase (GST) and p32, was constructed by amplifying the entire p32 coding sequence by PCR from an EBV-transformed human Article Title: Role of Host Cell p32 in Herpes Simplex Virus 1 De-Envelopment during Viral Nuclear Egress Article Snippet: Plasmids were constructed for this study as follows. (i) To construct pcDNA-MEF-UL47 (Fig. 2A), an expression plasmid for UL47 fused to an MEF tag (MEFUL47), the UL47 ORF without a start codon, was amplified by PCR from pBC1007 (52) and cloned into pcDNA-MEF (53). (ii) pcDNA-MEF-gB, an expression plasmid for gB fused to an MEF tag (MEF-gB), was constructed by cloning a DNA fragment encoding MEF-gB amplified by PCR from recombinant virus DNA expressing MEF-gB (53) into pcDNA4/ HisMax C (Invitrogen). (iii) pGEX-p32, for generating a fusion protein of glutathione S-transferase (GST) and p32, was constructed by amplifying the entire p32 coding sequence by PCR from an EBV-transformed human Polymerase Chain Reaction:Article Title: Role of Host Cell p32 in Herpes Simplex Virus 1 De-Envelopment during Viral Nuclear Egress Article Snippet: Plasmids were constructed for this study as follows. (i) To construct pcDNA-MEF-UL47 , an expression plasmid for UL47 fused to an MEF tag (MEF-UL47), the UL47 ORF without a start codon, was amplified by PCR from pBC1007 ( 52 ) and cloned into pcDNA-MEF ( 53 ). (ii) pcDNA-MEF-gB, an expression plasmid for gB fused to an MEF tag (MEF-gB), was constructed by cloning a DNA fragment encoding MEF-gB amplified by PCR from recombinant virus DNA expressing MEF-gB ( 53 ) into pcDNA4/HisMax C (Invitrogen). (iii) pGEX-p32, for generating a fusion protein of glutathione S -transferase (GST) and p32, was constructed by amplifying the entire p32 coding sequence by PCR from an EBV-transformed human Article Title: Role of Host Cell p32 in Herpes Simplex Virus 1 De-Envelopment during Viral Nuclear Egress Article Snippet: Plasmids were constructed for this study as follows. (i) To construct pcDNA-MEF-UL47 (Fig. 2A), an expression plasmid for UL47 fused to an MEF tag (MEFUL47), the UL47 ORF without a start codon, was amplified by PCR from pBC1007 (52) and cloned into pcDNA-MEF (53). (ii) pcDNA-MEF-gB, an expression plasmid for gB fused to an MEF tag (MEF-gB), was constructed by cloning a DNA fragment encoding MEF-gB amplified by PCR from recombinant virus DNA expressing MEF-gB (53) into pcDNA4/ HisMax C (Invitrogen). (iii) pGEX-p32, for generating a fusion protein of glutathione S-transferase (GST) and p32, was constructed by amplifying the entire p32 coding sequence by PCR from an EBV-transformed human Clone Assay:Article Title: Role of Host Cell p32 in Herpes Simplex Virus 1 De-Envelopment during Viral Nuclear Egress Article Snippet: Plasmids were constructed for this study as follows. (i) To construct pcDNA-MEF-UL47 , an expression plasmid for UL47 fused to an MEF tag (MEF-UL47), the UL47 ORF without a start codon, was amplified by PCR from pBC1007 ( 52 ) and cloned into pcDNA-MEF ( 53 ). (ii) pcDNA-MEF-gB, an expression plasmid for gB fused to an MEF tag (MEF-gB), was constructed by cloning a DNA fragment encoding MEF-gB amplified by PCR from recombinant virus DNA expressing MEF-gB ( 53 ) into pcDNA4/HisMax C (Invitrogen). (iii) pGEX-p32, for generating a fusion protein of glutathione S -transferase (GST) and p32, was constructed by amplifying the entire p32 coding sequence by PCR from an EBV-transformed human Article Title: Role of Host Cell p32 in Herpes Simplex Virus 1 De-Envelopment during Viral Nuclear Egress Article Snippet: Plasmids were constructed for this study as follows. (i) To construct pcDNA-MEF-UL47 (Fig. 2A), an expression plasmid for UL47 fused to an MEF tag (MEFUL47), the UL47 ORF without a start codon, was amplified by PCR from pBC1007 (52) and cloned into pcDNA-MEF (53). (ii) pcDNA-MEF-gB, an expression plasmid for gB fused to an MEF tag (MEF-gB), was constructed by cloning a DNA fragment encoding MEF-gB amplified by PCR from recombinant virus DNA expressing MEF-gB (53) into pcDNA4/ HisMax C (Invitrogen). (iii) pGEX-p32, for generating a fusion protein of glutathione S-transferase (GST) and p32, was constructed by amplifying the entire p32 coding sequence by PCR from an EBV-transformed human Cloning:Article Title: Role of Host Cell p32 in Herpes Simplex Virus 1 De-Envelopment during Viral Nuclear Egress Article Snippet: Plasmids were constructed for this study as follows. (i) To construct pcDNA-MEF-UL47 , an expression plasmid for UL47 fused to an MEF tag (MEF-UL47), the UL47 ORF without a start codon, was amplified by PCR from pBC1007 ( 52 ) and cloned into pcDNA-MEF ( 53 ). (ii) pcDNA-MEF-gB, an expression plasmid for gB fused to an MEF tag (MEF-gB), was constructed by cloning a DNA fragment encoding MEF-gB amplified by PCR from recombinant virus DNA expressing MEF-gB ( 53 ) into pcDNA4/HisMax C (Invitrogen). (iii) pGEX-p32, for generating a fusion protein of glutathione S -transferase (GST) and p32, was constructed by amplifying the entire p32 coding sequence by PCR from an EBV-transformed human Article Title: Role of Host Cell p32 in Herpes Simplex Virus 1 De-Envelopment during Viral Nuclear Egress Article Snippet: Plasmids were constructed for this study as follows. (i) To construct pcDNA-MEF-UL47 (Fig. 2A), an expression plasmid for UL47 fused to an MEF tag (MEFUL47), the UL47 ORF without a start codon, was amplified by PCR from pBC1007 (52) and cloned into pcDNA-MEF (53). (ii) pcDNA-MEF-gB, an expression plasmid for gB fused to an MEF tag (MEF-gB), was constructed by cloning a DNA fragment encoding MEF-gB amplified by PCR from recombinant virus DNA expressing MEF-gB (53) into pcDNA4/ HisMax C (Invitrogen). (iii) pGEX-p32, for generating a fusion protein of glutathione S-transferase (GST) and p32, was constructed by amplifying the entire p32 coding sequence by PCR from an EBV-transformed human Recombinant:Article Title: Role of Host Cell p32 in Herpes Simplex Virus 1 De-Envelopment during Viral Nuclear Egress Article Snippet: Plasmids were constructed for this study as follows. (i) To construct pcDNA-MEF-UL47 , an expression plasmid for UL47 fused to an MEF tag (MEF-UL47), the UL47 ORF without a start codon, was amplified by PCR from pBC1007 ( 52 ) and cloned into pcDNA-MEF ( 53 ). (ii) pcDNA-MEF-gB, an expression plasmid for gB fused to an MEF tag (MEF-gB), was constructed by cloning a DNA fragment encoding MEF-gB amplified by PCR from recombinant virus DNA expressing MEF-gB ( 53 ) into pcDNA4/HisMax C (Invitrogen). (iii) pGEX-p32, for generating a fusion protein of glutathione S -transferase (GST) and p32, was constructed by amplifying the entire p32 coding sequence by PCR from an EBV-transformed human Article Title: Role of Host Cell p32 in Herpes Simplex Virus 1 De-Envelopment during Viral Nuclear Egress Article Snippet: Plasmids were constructed for this study as follows. (i) To construct pcDNA-MEF-UL47 (Fig. 2A), an expression plasmid for UL47 fused to an MEF tag (MEFUL47), the UL47 ORF without a start codon, was amplified by PCR from pBC1007 (52) and cloned into pcDNA-MEF (53). (ii) pcDNA-MEF-gB, an expression plasmid for gB fused to an MEF tag (MEF-gB), was constructed by cloning a DNA fragment encoding MEF-gB amplified by PCR from recombinant virus DNA expressing MEF-gB (53) into pcDNA4/ HisMax C (Invitrogen). (iii) pGEX-p32, for generating a fusion protein of glutathione S-transferase (GST) and p32, was constructed by amplifying the entire p32 coding sequence by PCR from an EBV-transformed human Virus:Article Title: Role of Host Cell p32 in Herpes Simplex Virus 1 De-Envelopment during Viral Nuclear Egress Article Snippet: Plasmids were constructed for this study as follows. (i) To construct pcDNA-MEF-UL47 , an expression plasmid for UL47 fused to an MEF tag (MEF-UL47), the UL47 ORF without a start codon, was amplified by PCR from pBC1007 ( 52 ) and cloned into pcDNA-MEF ( 53 ). (ii) pcDNA-MEF-gB, an expression plasmid for gB fused to an MEF tag (MEF-gB), was constructed by cloning a DNA fragment encoding MEF-gB amplified by PCR from recombinant virus DNA expressing MEF-gB ( 53 ) into pcDNA4/HisMax C (Invitrogen). (iii) pGEX-p32, for generating a fusion protein of glutathione S -transferase (GST) and p32, was constructed by amplifying the entire p32 coding sequence by PCR from an EBV-transformed human Article Title: Role of Host Cell p32 in Herpes Simplex Virus 1 De-Envelopment during Viral Nuclear Egress Article Snippet: Plasmids were constructed for this study as follows. (i) To construct pcDNA-MEF-UL47 (Fig. 2A), an expression plasmid for UL47 fused to an MEF tag (MEFUL47), the UL47 ORF without a start codon, was amplified by PCR from pBC1007 (52) and cloned into pcDNA-MEF (53). (ii) pcDNA-MEF-gB, an expression plasmid for gB fused to an MEF tag (MEF-gB), was constructed by cloning a DNA fragment encoding MEF-gB amplified by PCR from recombinant virus DNA expressing MEF-gB (53) into pcDNA4/ HisMax C (Invitrogen). (iii) pGEX-p32, for generating a fusion protein of glutathione S-transferase (GST) and p32, was constructed by amplifying the entire p32 coding sequence by PCR from an EBV-transformed human Sequencing:Article Title: Role of Host Cell p32 in Herpes Simplex Virus 1 De-Envelopment during Viral Nuclear Egress Article Snippet: Plasmids were constructed for this study as follows. (i) To construct pcDNA-MEF-UL47 , an expression plasmid for UL47 fused to an MEF tag (MEF-UL47), the UL47 ORF without a start codon, was amplified by PCR from pBC1007 ( 52 ) and cloned into pcDNA-MEF ( 53 ). (ii) pcDNA-MEF-gB, an expression plasmid for gB fused to an MEF tag (MEF-gB), was constructed by cloning a DNA fragment encoding MEF-gB amplified by PCR from recombinant virus DNA expressing MEF-gB ( 53 ) into pcDNA4/HisMax C (Invitrogen). (iii) pGEX-p32, for generating a fusion protein of glutathione S -transferase (GST) and p32, was constructed by amplifying the entire p32 coding sequence by PCR from an EBV-transformed human Article Title: Role of Host Cell p32 in Herpes Simplex Virus 1 De-Envelopment during Viral Nuclear Egress Article Snippet: Plasmids were constructed for this study as follows. (i) To construct pcDNA-MEF-UL47 (Fig. 2A), an expression plasmid for UL47 fused to an MEF tag (MEFUL47), the UL47 ORF without a start codon, was amplified by PCR from pBC1007 (52) and cloned into pcDNA-MEF (53). (ii) pcDNA-MEF-gB, an expression plasmid for gB fused to an MEF tag (MEF-gB), was constructed by cloning a DNA fragment encoding MEF-gB amplified by PCR from recombinant virus DNA expressing MEF-gB (53) into pcDNA4/ HisMax C (Invitrogen). (iii) pGEX-p32, for generating a fusion protein of glutathione S-transferase (GST) and p32, was constructed by amplifying the entire p32 coding sequence by PCR from an EBV-transformed human cDNA Library Assay:Article Title: Role of Host Cell p32 in Herpes Simplex Virus 1 De-Envelopment during Viral Nuclear Egress Article Snippet: Plasmids were constructed for this study as follows. (i) To construct pcDNA-MEF-UL47 , an expression plasmid for UL47 fused to an MEF tag (MEF-UL47), the UL47 ORF without a start codon, was amplified by PCR from pBC1007 ( 52 ) and cloned into pcDNA-MEF ( 53 ). (ii) pcDNA-MEF-gB, an expression plasmid for gB fused to an MEF tag (MEF-gB), was constructed by cloning a DNA fragment encoding MEF-gB amplified by PCR from recombinant virus DNA expressing MEF-gB ( 53 ) into pcDNA4/HisMax C (Invitrogen). (iii) pGEX-p32, for generating a fusion protein of glutathione S -transferase (GST) and p32, was constructed by amplifying the entire p32 coding sequence by PCR from an EBV-transformed human Article Title: Role of Host Cell p32 in Herpes Simplex Virus 1 De-Envelopment during Viral Nuclear Egress Article Snippet: Plasmids were constructed for this study as follows. (i) To construct pcDNA-MEF-UL47 (Fig. 2A), an expression plasmid for UL47 fused to an MEF tag (MEFUL47), the UL47 ORF without a start codon, was amplified by PCR from pBC1007 (52) and cloned into pcDNA-MEF (53). (ii) pcDNA-MEF-gB, an expression plasmid for gB fused to an MEF tag (MEF-gB), was constructed by cloning a DNA fragment encoding MEF-gB amplified by PCR from recombinant virus DNA expressing MEF-gB (53) into pcDNA4/ HisMax C (Invitrogen). (iii) pGEX-p32, for generating a fusion protein of glutathione S-transferase (GST) and p32, was constructed by amplifying the entire p32 coding sequence by PCR from an EBV-transformed human FLAG-tag:Article Title: Role of Host Cell p32 in Herpes Simplex Virus 1 De-Envelopment during Viral Nuclear Egress Article Snippet: Plasmids were constructed for this study as follows. (i) To construct pcDNA-MEF-UL47 , an expression plasmid for UL47 fused to an MEF tag (MEF-UL47), the UL47 ORF without a start codon, was amplified by PCR from pBC1007 ( 52 ) and cloned into pcDNA-MEF ( 53 ). (ii) pcDNA-MEF-gB, an expression plasmid for gB fused to an MEF tag (MEF-gB), was constructed by cloning a DNA fragment encoding MEF-gB amplified by PCR from recombinant virus DNA expressing MEF-gB ( 53 ) into pcDNA4/HisMax C (Invitrogen). (iii) pGEX-p32, for generating a fusion protein of glutathione S -transferase (GST) and p32, was constructed by amplifying the entire p32 coding sequence by PCR from an EBV-transformed human Article Title: Role of Host Cell p32 in Herpes Simplex Virus 1 De-Envelopment during Viral Nuclear Egress Article Snippet: Plasmids were constructed for this study as follows. (i) To construct pcDNA-MEF-UL47 (Fig. 2A), an expression plasmid for UL47 fused to an MEF tag (MEFUL47), the UL47 ORF without a start codon, was amplified by PCR from pBC1007 (52) and cloned into pcDNA-MEF (53). (ii) pcDNA-MEF-gB, an expression plasmid for gB fused to an MEF tag (MEF-gB), was constructed by cloning a DNA fragment encoding MEF-gB amplified by PCR from recombinant virus DNA expressing MEF-gB (53) into pcDNA4/ HisMax C (Invitrogen). (iii) pGEX-p32, for generating a fusion protein of glutathione S-transferase (GST) and p32, was constructed by amplifying the entire p32 coding sequence by PCR from an EBV-transformed human Stable Transfection:Article Title: Role of Host Cell p32 in Herpes Simplex Virus 1 De-Envelopment during Viral Nuclear Egress Article Snippet: Plasmids were constructed for this study as follows. (i) To construct pcDNA-MEF-UL47 , an expression plasmid for UL47 fused to an MEF tag (MEF-UL47), the UL47 ORF without a start codon, was amplified by PCR from pBC1007 ( 52 ) and cloned into pcDNA-MEF ( 53 ). (ii) pcDNA-MEF-gB, an expression plasmid for gB fused to an MEF tag (MEF-gB), was constructed by cloning a DNA fragment encoding MEF-gB amplified by PCR from recombinant virus DNA expressing MEF-gB ( 53 ) into pcDNA4/HisMax C (Invitrogen). (iii) pGEX-p32, for generating a fusion protein of glutathione S -transferase (GST) and p32, was constructed by amplifying the entire p32 coding sequence by PCR from an EBV-transformed human Article Title: Role of Host Cell p32 in Herpes Simplex Virus 1 De-Envelopment during Viral Nuclear Egress Article Snippet: Plasmids were constructed for this study as follows. (i) To construct pcDNA-MEF-UL47 (Fig. 2A), an expression plasmid for UL47 fused to an MEF tag (MEFUL47), the UL47 ORF without a start codon, was amplified by PCR from pBC1007 (52) and cloned into pcDNA-MEF (53). (ii) pcDNA-MEF-gB, an expression plasmid for gB fused to an MEF tag (MEF-gB), was constructed by cloning a DNA fragment encoding MEF-gB amplified by PCR from recombinant virus DNA expressing MEF-gB (53) into pcDNA4/ HisMax C (Invitrogen). (iii) pGEX-p32, for generating a fusion protein of glutathione S-transferase (GST) and p32, was constructed by amplifying the entire p32 coding sequence by PCR from an EBV-transformed human shRNA:Article Title: Role of Host Cell p32 in Herpes Simplex Virus 1 De-Envelopment during Viral Nuclear Egress Article Snippet: Plasmids were constructed for this study as follows. (i) To construct pcDNA-MEF-UL47 , an expression plasmid for UL47 fused to an MEF tag (MEF-UL47), the UL47 ORF without a start codon, was amplified by PCR from pBC1007 ( 52 ) and cloned into pcDNA-MEF ( 53 ). (ii) pcDNA-MEF-gB, an expression plasmid for gB fused to an MEF tag (MEF-gB), was constructed by cloning a DNA fragment encoding MEF-gB amplified by PCR from recombinant virus DNA expressing MEF-gB ( 53 ) into pcDNA4/HisMax C (Invitrogen). (iii) pGEX-p32, for generating a fusion protein of glutathione S -transferase (GST) and p32, was constructed by amplifying the entire p32 coding sequence by PCR from an EBV-transformed human Article Title: Role of Host Cell p32 in Herpes Simplex Virus 1 De-Envelopment during Viral Nuclear Egress Article Snippet: Plasmids were constructed for this study as follows. (i) To construct pcDNA-MEF-UL47 (Fig. 2A), an expression plasmid for UL47 fused to an MEF tag (MEFUL47), the UL47 ORF without a start codon, was amplified by PCR from pBC1007 (52) and cloned into pcDNA-MEF (53). (ii) pcDNA-MEF-gB, an expression plasmid for gB fused to an MEF tag (MEF-gB), was constructed by cloning a DNA fragment encoding MEF-gB amplified by PCR from recombinant virus DNA expressing MEF-gB (53) into pcDNA4/ HisMax C (Invitrogen). (iii) pGEX-p32, for generating a fusion protein of glutathione S-transferase (GST) and p32, was constructed by amplifying the entire p32 coding sequence by PCR from an EBV-transformed human |